FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135426844
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2289280.1690418186220156No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2118070.15639956875905636No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1506770.11126080734776629No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1376800.10166374400632124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG874200.058.523285145
ACCGCGT492100.056.7649656
AGACCGC531100.052.2440874
CCGCGTT566650.049.2745177
CAGACCG584250.048.042973
GCGTTCT733100.037.9789969
CGGTGCT871750.033.68765145
CTCGCTA479000.031.8033081
TCGCTAT463250.031.3132672
CGCTATG463350.030.9711933
CCGCTAA363800.026.2279433
CTTCGAC1211400.025.726599145
CGCGTTC1086800.025.5576738
ACCGCTA366050.025.4628772
GACCGCG1155750.024.3008295
GTGCTCT1313750.023.92969145
CGTATCG169000.023.0326543
TCGCGTC327850.022.7196859
CGCTAAG411400.022.5942734
CGATCCT986350.021.764019145
Produced by FastQC (version 0.11.5)