FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135426844
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1048200.033.3748324
CGGAGGG1233300.029.0064851
GCGACAT522900.028.421337
TGCGACA526300.028.032966
CAATCGC362100.024.8559131
CGGCCGC4045200.024.78501145
GAGGGCG1441800.024.6687553
GGGCGAT318000.024.66281
GGCGATC329100.023.878662
CTACCGT328650.023.4489548
CGACATC671800.022.3296458
GCGAGGC1573050.022.295487
GGGCGAG1648850.021.945245
TCAATCG267250.021.5841873
ACTACCG364000.021.5719877
TACCGTA376400.020.8278129
GGCGAGG1803950.020.1583736
CTGCGAC793050.018.9332835
GGTCACG605450.018.097004145
CGAGGCC1975900.018.032238
Produced by FastQC (version 0.11.5)