FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118406522
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2154500.18195788235381155No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1724860.14567271894026243No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1624120.13716474165164652No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1415250.11952466604837865No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1316870.11121600210501918No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1249600.10553472721713758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT423300.051.589556
CCGCGTT456700.047.816637
AGACCGC459150.047.3741044
TCGACCG596200.045.10906145
CAGACCG506550.043.6428833
GTGCTCT1122850.040.317856145
CTCGCTA518850.039.011721
TCGCTAT564450.035.572912
CGCTATG611400.032.8503953
GCGTTCT666900.032.5523269
CGCGTTC696300.031.089848
GACCGCG761550.028.8284515
ACCGCTA194300.026.5292022
CCGGTAA163600.025.380231
CGATCCT1221150.025.18446145
CGTATCG133150.024.4883793
CTTCGAC851650.023.400772145
CCGCTAA234250.022.9461383
CGGTGCT725100.022.335058145
CGGTAAT180900.020.8709952
Produced by FastQC (version 0.11.5)