FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118561905
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1549080.13065579538385452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG169300.027.1715073
CAATCGC253400.025.5943181
TGGCGCG1019700.021.4996264
CGGTGGC1735000.021.2803541
TGCGACA325500.018.154876
AGGGCGA768250.017.9374624
AATCGCC365350.017.6852532
GCGACAT348200.017.0067657
GTGGCGC1388400.016.857353
CGGAGGG939600.015.2363591
GGGGACC4415950.015.175187145
GGCGATC264000.015.0522622
ATATCGG232400.014.342085145
GGCGCGT1403450.013.9293865
AGGGATC736350.013.8926932
CGGCCGC3298650.013.773945145
GCCGTAT259000.013.696953145
GGGCGAT289750.013.6209271
GGTCACG590950.013.226531145
GGTGGCG2283800.013.211972
Produced by FastQC (version 0.11.5)