FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126453561
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3203460.253330944155855No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2357610.18644077567732553No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2072450.16389020472108334No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2071600.16382298636888526No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2061040.16298789719334197No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2048720.1620136264885415No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1947700.154024923030835No Hit
CACCGCTAAGAGTCGTACGAGGTCGATTTGGCGAGGGCGCTCCCGACGAC1878870.14858181811107715No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1817000.1436891128752001No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1726350.13652047331431022No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1720160.13603096554947947No Hit
CCACACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCGGCCGGCCG1500360.11864909047519824No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1445410.11430362170662793No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1439800.11385998058212057No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1434390.11343215554048336No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1415740.11195730581284302No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1386330.10963155082678928No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1357450.10734770846034143No Hit
CTGGAGGCGACAGCGAGGGCTGTCTGCCGCGTCAGAGGACCCCGCCGGCC1329060.10510261549692539No Hit
CCACCGCTAAGAGTCGTACGAGGTCGATTTGGCGAGGGCGCTCCCGACGA1320580.10443201358323155No Hit
CTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCTCTCAGCC1298110.1026550766727716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA546350.045.2833252
ACCGCGT411650.040.5653276
CCGCTAA671150.039.0887763
AGACCGC421050.038.436884
CTCGCTA1080850.038.0328641
CAGACCG475750.034.9465833
CCGCGTT478300.034.8620077
TCGCTAT1194950.034.6794932
CACCGCT726100.034.463611
CGCTAAG768450.034.1945534
CGCTATG1294050.032.199313
GTCTCGC916950.031.6610834
TCGACCG834250.030.915178145
GGGGTCT1545500.028.8435571
GGTGCTC1055400.028.296738145
AGAGTCG957050.027.8233179
GCGTTCT603250.026.8582129
CGATCCT2007800.026.747894145
TCTCGCT1127700.025.8192735
CTTCGAC1016600.025.222225145
Produced by FastQC (version 0.11.5)