FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126453561
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1562200.1235394232986448No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1536670.12152050032027172No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1465600.11590025527236833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG290000.041.2444463
CAATCGC435100.034.555511
AGGGCGA1181700.030.8932724
CGGAGGG1414350.025.8880421
TGGCGCG1608750.025.4203264
GATCAAT505050.024.6279721
AATCGCC616350.024.5326772
GGCGATC313950.022.601872
GGGCGAT322900.022.4752481
ATCAATC568500.022.1359652
CGGTGGC2646800.020.598191
GTGGCGC2061200.020.2107353
TGCGACA400400.020.0423816
GGGCGAG1880650.019.7951725
GCGACAT420900.019.5018467
GGCGAGG1984000.018.8071356
GAGGGCG2024000.018.5710833
GCGAGGC1951050.018.357267
CGGCCGC6599850.016.034779145
GGTCACG957850.015.961993145
Produced by FastQC (version 0.11.5)