FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138723935
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3150490.22710500534749103No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2676430.19293209928048827No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2282360.16452532145948714No Hit
CACCGCTAAGAGTCGTACGAGGTCGATTTGGCGAGGGCGCTCCCGACGAC1953330.1408069919585254No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1936010.1395584691279122No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1880630.1355663678369562No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1702220.12270557348304746No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1644730.11856137154702251No Hit
CTCCGTCTTCGCGGGCGGCGGCGGCGCCGCCCTCCCCGTCTCTCTCAGCC1628500.11739142203542596No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1572460.1133517442393773No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1530970.11036091212378023No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1468650.1058685366732136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT578700.051.1371276
AGACCGC614250.047.6955384
ACCGCTA568150.046.9545752
CCGCGTT648000.045.724787
CAGACCG670050.044.2891583
GTGCTCT1372450.042.05112145
TCGACCG806600.039.633408145
CCGCTAA732450.038.2993773
CTCGCTA920400.037.573141
GCGTTCT834600.035.1507769
TCGCTAT981850.035.0665862
CGCTAAG849800.033.103334
CGCTATG1063650.032.5769043
CACCGCT873900.030.8656141
GTCGCGT116850.030.7997631
AGAGTCG1006300.028.5105789
CGATCCT1943000.026.008867145
CTTCGAC1100350.024.151009145
GTCTCGC835950.023.889924
TCAGACC1372500.022.7372552
Produced by FastQC (version 0.11.5)