FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138723935
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2953080.2128745843318242No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG2243950.16175651303432245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG306750.041.273523
CAATCGC494650.035.7619671
AGGGCGA1113300.033.0754054
CGGAGGG1385050.027.0741
AATCGCC730400.024.3254872
TGGCGCG1465600.024.1445664
GATCAAT561150.023.54881
GGCGATC317600.022.9164352
GGGCGAT325750.022.3628791
CGGTGGC2549550.021.6488061
ATCAATC635000.021.3028872
TGCGACA472550.021.1029556
GGGCGAG1785300.020.8511055
GCGACAT487500.020.7070987
GTGGCGC1861600.020.1716423
TACCGTA210150.020.1503669
GCGAGGC1847850.019.6765797
CGGCCGC6011500.019.588669145
GGCGAGG1989000.018.9439816
GAGGGCG2029450.018.6517663
Produced by FastQC (version 0.11.5)