FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122822754
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1756940.1430467843116431No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1256720.10231980305538499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1050250.033.3223464
CAATCGC357800.032.1416361
TCAATCG226650.029.898023
GCGACAT454350.023.8371147
AATCGCC486150.023.621042
TGCGACA489850.022.0711566
CGGAGGG1653850.021.5318851
GGGCGAG1804200.020.0466545
GGCGAGG1804000.020.0195836
TCGCGTC246300.019.571519
CTCGCGT245250.019.3249958
GGGCGAT328950.019.2436431
GGCGATC335200.019.1563572
GCGAGGC1786150.019.0692947
GAGGGCG1968100.018.152283
CGACATC627350.017.5416498
TACCGTA164100.016.5092959
CGGCCGC6229400.016.014378145
GATCAAT454100.015.786741
GCTGCGA710400.015.6058094
Produced by FastQC (version 0.11.5)