FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127981645
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC306300.025.6538981
TCAATCG207250.025.329023
TGCGACA432750.024.404056
GCGACAT447350.024.0149237
GGCGATC300900.023.2065942
AGGGCGA820050.023.1934454
GGGCGAT297300.023.1801511
TACCGTA218200.022.4738929
CGGAGGG873150.022.0906561
CTACCGT260200.019.1356778
CGACATC608750.017.854028
CGGCCGC3169200.017.695072145
CGGTGGC1654600.016.9559751
GCTGCGA635150.016.9548554
AATCGCC477650.016.75092
ACTACCG310100.016.4209297
CTGCGAC684050.015.8623715
GGGCGAG1239150.015.6989195
GAGGGCG1238900.015.5042183
GGGGACC3306150.015.032513145
Produced by FastQC (version 0.11.5)