FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105894020
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1211690.11442478054945879No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1088280.10277067581342175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA788850.034.799274
TGCGACA415100.031.4313436
CAATCGC342600.031.2170621
TCAATCG225100.030.8147933
GCGACAT441650.029.7686427
CGACATC578200.022.7911748
TGGCGCG1243900.022.411054
TACCGTA180650.021.9012229
AATCGCC494900.021.6990872
CGGTGGC2032050.021.2912251
GCTGCGA624050.021.056594
GGGCGAG1350500.020.9954745
CTGCGAC637900.020.7182755
CGGAGGG1430300.020.3353921
GGGCGAT280850.020.2672961
GGCGAGG1398050.020.173916
GGCGATC285450.020.1156482
GCGAGGC1405300.019.2812947
GTGGCGC1590550.019.0932083
CTACCGT209400.019.043888
Produced by FastQC (version 0.11.5)