FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108669740
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA689900.032.9360124
CAATCGC290150.025.706261
TGCGACA361650.024.4698336
GCGACAT365200.024.2690247
GCGAGGC929050.024.2019027
ACGATAC120550.023.5743755
GGGCGAG996850.023.3241025
GGCGAGG1037200.022.3530086
TCGCGTC237450.021.4872329
CTCGCGT237900.021.4465878
CGGAACG134950.021.2082141
TACCGTA156500.021.1994539
ATACGGC138350.020.9710738
TGGCGCG774300.020.2441674
GGCGATC335150.019.6734312
GGGCGAT340500.019.4363271
AACGATA156100.018.5078584
CGACATC504800.017.9364538
CGATCTG371850.017.9035594
CGAGGCC1334800.017.4038168
Produced by FastQC (version 0.11.5)