FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105999814
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2087110.1968975153107344No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1768710.16685972675386015No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1360880.12838513093994675No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1335560.12599644750320035No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1062010.10018979844625009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT468300.058.88046
CCGCGTT502350.054.816177
AGACCGC519500.052.847324
CAGACCG551200.050.4730873
GCGTTCT651300.042.1836629
TCGACCG306700.042.105225145
GTGCTCT1090200.040.594223145
CGCGTTC696300.039.5024578
CTCGCTA605150.038.670131
GACCGCG779200.035.4751365
TCGCTAT667450.034.98562
CGCTATG730150.032.135833
ACCGCTA196200.027.7972622
CGATCCT1277300.026.64281145
TCAGACC1107150.026.3456172
CCGCTAA229350.025.118783
CTCAGAC1357900.024.2840651
CGCTAAG258600.021.6934644
GCTATGT1168250.020.420554
AACGCTA566100.019.769974115-119
Produced by FastQC (version 0.11.5)