FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105999814
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1284750.12120304286571673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC264600.030.3274881
TCAATCG178300.027.7892593
TGGCGCG894350.025.8607464
CGGTGGC1574950.023.1658631
GTGGCGC1181950.020.629713
AATCGCC413450.019.546582
AGGGCGA596400.019.2368774
GGCGCGT1167600.018.6033735
GGGGACC3860800.016.72304145
CGTGCCT1374250.016.2783669
GGTGGCG2054500.015.4704312
GCGACAT251700.015.1218397
ACGCCCG709300.014.9255026
GCGTGCC1415650.014.9120428
TGCGACA248950.014.8838346
TACCGTA153000.014.7860179
GGTCACG688550.013.969596145
CGGAGGG899850.013.8354421
ATATCGG219900.013.610382145
GGGCGAG912900.013.2670595
Produced by FastQC (version 0.11.5)