FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116708821
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2146160.18389012772222246No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1998280.17121927741862802No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1721990.14754583117586287No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1707430.14629828194391578No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1619670.13877871322168528No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1509400.12933041282286623No Hit
CTGGAGGCGACAGCGAGGGCTGTCTGCCGCGTCAGAGGACCCCGCCGGCC1499660.1284958572240225No Hit
CACCGCTAAGAGTCGTACGAGGTCGATTTGGCGAGGGCGCTCCCGACGAC1361960.11669726318287459No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1300810.11145772777534955No Hit
CCACACACGACCGGTCGGAGGCAGAACGGCAGCCCCTCGGCGGCCGGCCG1263440.10825574186890295No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1200590.10287054480654895No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1191180.1020642647054073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT492200.049.8530436
AGACCGC495600.048.566644
ACCGCTA386250.048.1955642
CCGCGTT555950.044.0817267
CAGACCG560600.043.630053
TCGACCG995850.039.762844145
CTCGCTA712650.038.829161
CCGCTAA519450.038.6823653
GTGCTCT1091600.037.813015145
GCGTTCT704050.034.2332239
TCGCTAT825600.033.7123032
CGCTAAG620850.032.3408364
CACCGCT587800.032.019421
CGCTATG911400.030.6343943
CGATCCT1566300.026.168184145
CTTCGAC1209100.024.72198145
TCAGACC1091800.023.3563632
GTCTCGC682600.023.1484744
CTCAGAC1332700.022.2722531
GCTATGT1271100.022.026664
Produced by FastQC (version 0.11.5)