FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116708821
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1661090.14232771659993035No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCGCGCTTCCCTCCGCAC1330110.11396824923799033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA974650.032.2900474
TCAATCG241500.031.8260883
CAATCGC378300.031.7556111
TGCGACA473050.024.8865456
GGCGATC312000.024.031352
GCGACAT507350.023.5378637
AATCGCC551150.022.0166652
GGGCGAT347650.021.7563531
CGGAGGG1566950.020.762461
CTACCGT245650.020.5789788
GGGCGAG1700850.019.2415265
TTAACGG106250.019.073833
GGCGAGG1786400.018.2781756
TACCGTG281500.018.0918589
CGACATC670350.017.9453758
TGGCGCG1336400.017.5869754
ATCAATC486250.016.9809172
GAGGGCG1924400.016.9768283
GCGAGGC1855650.016.8322057
GCTGCGA720000.016.5744024
Produced by FastQC (version 0.11.5)