FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149611751
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1753090.11717595631909956No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1665510.11132213805852723No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1520170.1016076604838346No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1498720.10017394957164828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT611300.040.6796046
CCGCGTT656700.037.9061287
AGACCGC674050.036.5688824
CAGACCG717400.034.738713
CTCGCTA745450.032.891281
TCGCTAT757600.032.300912
TCGACCG765450.032.044846145
ACCGCTA260750.031.7888282
GTGCTCT1448850.030.497055145
CGCTATG802750.030.4879553
CTTCGAC1093250.029.56342145
GCGTTCT848250.029.3309579
CGGTGCT934050.028.005266145
CCGCTAA334900.027.1602763
CGCTAAG360400.024.3750884
CGCGTTC1047050.023.607168
CGATCCT1603200.021.388033145
GTCTCGC947550.020.7931464
GTCGCGT99400.020.616051
CGTATCG185300.020.257923
Produced by FastQC (version 0.11.5)