FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132939305
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA4740410.35658453306943344No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG2055030.15458407880197658No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1983890.14923276453115203No Hit
CCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGG1746070.13134339765052933No Hit
CCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGG1407060.10584228644793953No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1338400.10067752347584487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCT1636250.073.63353145
ACCGCGT949750.066.978766
AGACCGC973050.065.19474
CCGCGTT1007700.063.1535267
CAGACCG1036450.061.607213
GCGTTCT1205850.052.6885839
TCGACCG858950.049.327248145
CGCGTTC1387450.045.738748
GACCGCG1532600.041.6616485
TCAGACC1838000.036.0743522
CTCGCTA489150.033.563771
CTCAGAC2115300.033.482551
TCGCTAT521350.031.1409742
ACCGCTA192600.030.0182692
CGCTATG560450.028.8558163
CTTCGAC1131450.025.370981145
CCGCTAA250550.024.6717223
CGATCCT970550.023.110376145
TTCGGTG1120700.022.609173140-144
CGGTGCT1249100.021.679892145
Produced by FastQC (version 0.11.5)