FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95951441
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1476780.15390910075024303No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1328830.13848984300298314No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1195430.12458697728156057No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT965510.10062485669183437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT331950.054.8822336
CCGCGTT356550.051.150447
AGACCGC384850.047.2368434
TCGACCG420100.046.96038145
CAGACCG410450.044.956923
CTCGCTA473100.038.223141
GCGTTCT497050.036.7966589
CGCGTTC495100.036.7180148
TCGCTAT519050.034.705862
CGCTATG553450.032.4821623
GTGCTCT934300.032.321594145
GACCGCG573350.031.9339355
ACCGCTA141000.031.0503862
CTTCGAC628700.024.765947145
CGATCCT1037300.024.51979145
CCGCTAA194050.024.460813
CGCTAAG219900.021.1708224
TCAGACC951900.020.621712
TCGCGTC615650.020.1473459
CTCAGAC1197400.019.8927041
Produced by FastQC (version 0.11.5)