FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95951441
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1407400.023.527041
TGCGACA282650.023.4140856
GCGACAT289200.023.4057257
TGGCGCG788400.022.6444934
GTGGCGC1050900.022.346043
CAATCGC226250.022.1408331
TACCGTA149750.020.2641229
AGGGCGA508150.020.0632254
TCAATCG162600.017.9374583
GGTGGCG1659700.017.3328972
GGCGCGT940000.017.1767025
TCGCGTC174300.016.9221619
CTACCGT183650.016.7016188
CGACATC415700.016.5505498
CTCGCGT178700.016.5420978
GGGGACC3378200.016.436766145
GGCGATC252450.015.7497992
GGGCGAT258950.015.2414421
CGTGCCT1119750.014.9752349
CTGCGAC462500.014.7758035
Produced by FastQC (version 0.11.5)