FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100409924
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA650150.029.3870434
GCGACAT395050.028.6152947
TGCGACA401150.027.9982096
TGGCGCG1027400.025.297294
CGGTGGC1984050.024.9333921
CAATCGC281300.024.7966861
TCAATCG203400.024.5953773
GGGCGAT290200.024.2186471
TACCGTA146450.023.5060529
GGCGATC313500.022.566492
CGACATC507900.022.3216638
TCGCGTC209700.021.6692839
CTCGCGT211600.021.4437248
GTGGCGC1329200.020.6682453
GCTGCGA549700.020.539384
CTGCGAC587150.019.5419965
CTACCGT187500.018.3947668
GGGCGAG1104650.017.8479615
GCGAGGC1055250.017.7155597
CGTGCCT1612500.017.5505819
Produced by FastQC (version 0.11.5)