FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115578971
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1779040.15392419439345933No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1692980.146478203201861No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1248140.10799023292913726No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1223150.10582807490127248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG495750.053.259365145
ACCGCGT279450.049.906336
CCGCGTT319000.043.6396837
AGACCGC320400.043.0349924
CTCGCTA473250.042.0611841
CAGACCG367800.038.1471143
TCGCTAT524700.037.689792
CGCTATG577150.034.263943
ACCGCTA160500.033.8079722
GCGTTCT462850.030.0358879
CGATCCT1209450.026.270226145
CCGCTAA226600.026.2173253
GTGCTCT1008200.026.112234145
CTTCGAC665500.024.116964145
CGCGTTC595050.023.1718648
GACCGCG630550.022.2279015
CGCTAAG262650.022.1965164
CGCTAGA239750.019.499511
CCCGTAT163100.018.887371
CGTATCG77650.018.8014323
Produced by FastQC (version 0.11.5)