FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115578971
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1452600.12568030217192364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA645700.024.6586674
GCGACAT315350.024.4002657
TGGCGCG871700.023.9467434
TGCGACA323900.023.384376
CAATCGC214000.022.664281
CGGTGGC1415800.022.3261851
TCAATCG148300.022.1919613
CGGAGGG830300.019.7187581
GTGGCGC1180850.019.256843
CGGCCGC2372550.018.211023145
CGACATC433700.018.0340988
GGGGACC3603850.017.585238145
GGTGGCG1739250.016.3743042
GGCGCGT1192300.016.2668935
GGGCGAG1034750.016.0362555
GGCGATC236000.015.9679692
GGGCGAT233250.015.9254151
CTGCGAC489200.015.8974275
AATCGCC312700.015.8249372
GCGAGGC1004950.015.7990867
Produced by FastQC (version 0.11.5)