FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107403011
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1855180.17273072539837825No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1436650.1337625441431991No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1218300.11343257406442729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT408050.061.6807986
CCGCGTT444500.056.8132367
AGACCGC445900.056.215944
TCGACCG444450.055.9202145
CAGACCG497250.050.9767843
CTCGCTA398450.040.9858361
GCGTTCT639050.039.4457869
TCGCTAT429250.037.590982
CGCGTTC671400.037.3512428
CGCTATG463150.034.8358653
GACCGCG774600.032.551545
ACCGCTA161100.032.145432
CGATCCT910400.025.319689145
CCGCTAA223550.025.0389523
GTGCTCT1027000.024.054049145
TCAGACC1179650.023.0611672
CTTCGAC667350.022.810673145
TCGCGTC235650.021.7700219
CTCAGAC1453150.021.7698141
CGCTAAG251050.021.7499644
Produced by FastQC (version 0.11.5)