FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107403011
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1194540.11122034558230401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA634800.029.411534
CAATCGC350100.027.7348441
GCGACAT308950.025.2086857
TGCGACA318500.024.1860226
TACCGTA169550.023.75689
CGGAGGG850250.022.6993751
TGGCGCG827150.021.4975284
TCAATCG251900.021.3520413
GGGCGAG966500.019.872635
GCGAGGC927100.019.8713957
CGGCCGC2607150.019.160866145
CGGTGGC1399200.019.1399461
CTACCGT212650.019.125968
AATCGCC510800.018.9967542
GGCGAGG1028600.018.7543856
CGACATC438150.017.9690488
GTGGCGC1107850.017.3964863
GAGGGCG1106300.017.2259183
ACTACCG241200.017.078517
GGTCACG558800.017.036434145
Produced by FastQC (version 0.11.5)