FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140890250
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2135740.15158891406609046No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1874250.1330290775976336No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1760710.12497032264475363No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1622310.11514707369743471No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1506030.10689384112811214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG678800.050.279953145
ACCGCGT330450.044.370896
CTCGCTA599950.043.431081
TCGCTAT645850.040.0217862
CCGCGTT375650.039.066877
CGCTATG692000.037.205053
AGACCGC402950.035.9971164
CAGACCG461850.032.1300963
ACCGCTA190850.027.5889342
CTTCGAC987650.026.383146145
GCGTTCT592450.024.803339
CGATCCT1613700.024.579388145
CGCGTTC601950.024.1941038
CCGCTAA264400.022.3109253
GACCGCG665700.022.1042395
TTCGGCG291600.021.5335647
GTGCTCT1222000.019.578726145
CGCTAAG291300.019.5081484
TCGCGTC833100.019.3379659
CTCGCGT840200.019.1355448
Produced by FastQC (version 0.11.5)