FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140890250
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC405500.027.1780911
AGGGCGA840450.026.5274454
GCGACAT454700.025.2503597
TGCGACA461400.024.8118786
TGGCGCG1152450.024.7890194
TACCGTA229150.023.1719979
CGGTGGC2115200.023.0085531
GTGGCGC1501950.021.2123473
TCAATCG287050.021.111873
CTCGCGT284250.020.4938558
TCGCGTC285300.020.392739
CGACCGT116450.018.8938671
CTACCGT297750.018.361028
GGGCGAT393100.018.1819551
GCGAGGC1235550.018.1050647
AATCGCC611400.018.0637252
GGGGACC5368850.018.016161145
GGGCGAG1285050.017.9745925
GGCGATC402100.017.4239252
CGACATC691700.016.95678
Produced by FastQC (version 0.11.5)