FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180083306
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA4189810.23265954479978285No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2179910.121050087785483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT959600.058.8200046
AGACCGC1022300.054.9576574
CCGCGTT1029600.054.8147967
CAGACCG1078450.052.497153
GCGTTCT1322850.042.575369
TCGACCG875800.042.30658145
GTGCTCT1808700.038.909603145
CTCGCTA728800.035.782111
CGCGTTC1653100.034.0154468
TCGCTAT777250.033.0672872
ACCGCTA369350.032.7077832
CGCTATG827550.031.1836283
CCGCTAA472050.027.6100833
GACCGCG2093150.027.0600595
TCAGACC2256500.026.8954222
CGCTAAG498200.025.9311454
CCGGTAA243400.025.8488581
CTCAGAC2525050.025.5453821
GTCGCGT105900.024.077361
CGATCCT1565100.023.388998145
Produced by FastQC (version 0.11.5)