FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180083306
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1243550.030.819544
TCAATCG351200.024.1098373
TACCGTA334400.023.6962729
TCGCGTC387650.022.435879
CTCGCGT383950.022.403068
CAATCGC483250.021.7956711
TGCGACA538450.021.6003766
CTACCGT389100.020.7762098
GCGACAT573150.020.6495087
GGGCGAG2026250.019.7684615
GGGCGAT489000.019.3694691
ACTACCG436050.018.3926567
TGGCGCG1653800.018.3593544
GGCGATC511750.018.3282552
GGCGAGG2192450.018.2465156
GCGAGGC2119850.018.1901877
ACGATAC159500.017.0681255
GGTCACG829600.015.522814145
GTCGGTC305950.015.2982591
CGAGGCC2590250.015.2494468
Produced by FastQC (version 0.11.5)