FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116630764
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG1921060.16471297401430038No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCC1912730.16399875422234222No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1790870.15355039601729778No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1503800.12893682150620225No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1452650.12455118616902826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT313800.054.1420946
TCGACCG638950.051.247288145
CCGCGTT340100.049.9165847
AGACCGC402950.041.948164
CAGACCG415850.041.3232963
CTCGCTA548050.039.0751841
TCGCTAT586750.036.1233182
GCGTTCT484950.034.9689569
CGCGTTC493400.034.144748
CGCTATG635500.033.4018673
GACCGCG590350.028.9453435
ACCGCTA179400.028.5053652
CGATCCT1240450.026.225658145
CCGCTAA225700.024.6933293
TTCGGCG360000.024.5959137
GTGCTCT1079250.024.074799145
GTCGGCT310550.023.7606891
CTTCGAC844050.022.423193145
TCGCGTC1635850.021.7766489
TCGGCGA407100.021.703588
Produced by FastQC (version 0.11.5)