FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116630764
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG989650.026.8470844
GCGACAT373850.026.6916037
TGCGACA384500.025.7815636
CAATCGC268500.024.7858891
TCGCGTC321150.023.1457639
CTCGCGT323400.023.0045328
GTGGCGC1315850.021.6395053
GGCGCGT1272550.020.1874335
CGGTGGC1852250.019.6063291
AGGGCGA610200.018.6515584
CGACATC553100.018.361488
CGTGCCT1501150.017.7483679
TCAATCG240550.017.6780243
GCGTGCC1560950.016.3115438
CTGCGAC635800.016.0278455
AATCGCC411400.015.90693952
GCTGCGA643850.015.6888884
TACCGTA142250.015.404369
CGATCTG370000.015.3444954
GGCGATC377250.015.0502122
Produced by FastQC (version 0.11.5)