FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111760680
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1230160.110070912238544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG1097350.031.0426854
CAATCGC305650.029.6954211
AGGGCGA706300.027.8776264
TCAATCG220550.025.5203743
GTGGCGC1401450.025.0265963
CGGTGGC2059700.021.9009571
AATCGCC427300.021.17212
GGCGCGT1626250.019.9473175
CGTGCCT1680750.019.5074489
GCGAGGC1039000.018.8164567
GGGCGAG1139150.017.9581225
TCGCGTC265400.017.8491219
CTCGCGT269050.017.5093448
GGCGAGG1182450.017.2529496
GCGACAT230850.017.1757417
GCGTGCC1911100.016.6456958
GGTGGCG2520950.016.4735682
GGGGACC5041400.015.877614145
TGCGACA250100.015.43506056
ACGCCCG1407300.014.6172566
Produced by FastQC (version 0.11.5)