FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005788042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138890636
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1984010.14284692309998492No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1927670.1387904941266163No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1485900.1069834542337325No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1428880.10287806587623374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT464150.049.7117546
TCGACCG647350.048.864803145
CCGCGTT508700.045.409797
AGACCGC509200.044.9268884
CAGACCG567350.041.1201443
CTCGCTA652450.038.8202061
TCGCTAT693250.036.118682
GCGTTCT668400.034.3922589
CGCTATG740000.033.619263
GTGCTCT1331300.028.65119145
ACCGCTA214750.026.6272322
CTTCGAC943400.026.338017145
CGCGTTC879250.026.0402668
CGATCCT1450000.025.135859145
GACCGCG954450.024.2711185
CCGCTAA298500.020.2479633
CGCTAGA388500.019.514141
GTCGCGT91600.019.3889221
TCGCGTC385000.019.2949079
GCTATGT1362900.018.6439824
Produced by FastQC (version 0.11.5)