FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138890636
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1403320.10103776902569588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC363850.029.4030671
AGGGCGA878950.028.4241584
TCAATCG256150.026.2209573
TGCGACA440850.024.2535976
GCGACAT453150.024.2006577
TGGCGCG1206700.021.920524
CGGTGGC2027750.020.6714481
CGGAGGG1272450.020.5192091
GGCGATC339850.019.7634942
GGGCGAT345650.019.280181
AATCGCC555600.019.2436452
GGGCGAG1379850.018.7434655
GCGAGGC1347350.018.5772257
GGCGAGG1465300.017.5838346
CGACATC629350.017.3940878
GTGGCGC1618550.017.2884033
GAGGGCG1495750.017.1891543
CGGCCGC3630900.017.10803145
TACCGTA201150.016.8525269
GGGGACC4860100.016.323315145
Produced by FastQC (version 0.11.5)