FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122717115
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1622710.13223175919675098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1641450.024.8943541
TGGCGCG1087600.023.6589974
TCAATCG181800.022.9903473
TGCGACA390400.022.7342346
CAATCGC253650.022.5167981
AGGGCGA660350.022.4814034
TACCGTA181950.021.4161059
GCGACAT424650.021.2827957
GGGCGAT260600.020.234231
GTGGCGC1394450.019.9627533
GGCGATC272000.019.5299932
GGCGCGT1333500.018.124485
CGGAGGG847600.017.7661
CTACCGT219200.017.719398
GGGGACC4340950.017.55304145
CGACATC559950.016.2861738
ACTACCG250650.016.0978627
CGTGCCT1653700.015.7759999
GCTGCGA576650.015.640744
GGTGGCG2106100.015.1366212
Produced by FastQC (version 0.11.5)