FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115864685
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1530050.13205490525434907No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1529220.13198326996703094No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1424290.12292701611366742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG220700.035.2131653
AGGGCGA914850.034.325584
CAATCGC336800.031.4517291
TGCGACA370200.022.8558756
AATCGCC488400.021.8268092
TACCGTA153200.021.7972479
GCGACAT400700.021.4230337
GGGCGAG1595250.020.0917425
GATCAAT403550.020.0498681
CGGAGGG1666500.019.8675731
CGGTGGC2543550.018.7208081
GGCGAGG1747250.018.4466726
ATCAATC452750.018.334482
TGGCGCG1443600.018.0078934
GCGAGGC1771500.017.4243057
GAGGGCG1908200.017.2374553
TGCCGGT464250.016.9663289
CTACCGT200200.016.9590458
GGTCACG742000.016.935919145
GGCGATC307600.016.2078292
Produced by FastQC (version 0.11.5)