FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104639040
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1631010.1558701226616758No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1491010.1424907950225843No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1327480.12686278467386553No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1061450.10143919516081189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT355500.063.365496
CCGCGTT401450.056.128827
AGACCGC405600.055.203064
CAGACCG447900.050.814373
TCGACCG251900.049.40623145
CTCGCTA411950.044.483021
GCGTTCT515100.043.644099
CGCGTTC532500.042.120618
GACCGCG578000.038.8833735
TCGCTAT474350.038.3093762
CGCTATG482100.037.5445483
GTGCTCT1017100.031.516958145
CGATCCT986800.025.963667145
TCAGACC1037450.023.4817852
CTCAGAC1295400.022.1737791
ACCGCTA163700.020.6655882
GCTATGT926750.019.7056834
CCGCTAA178400.019.6887233
TTCGCGG518250.019.4698228
GCGGACG971350.018.94783145
Produced by FastQC (version 0.11.5)