FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104639040
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1564840.14954647901968518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC240450.028.1566241
TCAATCG172100.023.9689463
AGGGCGA576900.021.6648774
CGGTGGC1295900.020.0309981
AATCGCC367950.018.5778062
GCGACAT219150.017.991297
TGCGACA223150.017.4939426
CTACCGT196750.016.5070868
TGGCGCG689750.016.4722983
GGTCACG550450.016.360132145
ACGCCCG781650.016.3124086
GGGGACC3128400.016.296202145
TACCGTA203950.016.11819
GCGAGGC810450.015.3081347
GGGCGAG851300.015.1156185
CGGAGGG881600.015.07432651
TCGCGTC121300.014.9427469
CTCGCGT122450.014.7999588
GGTGGCG1577700.014.29544452
GGCGAGG907750.014.1537066
Produced by FastQC (version 0.11.5)