FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175358679
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA2797940.1595552621607055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2417880.13788196933212526No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2191940.12499752008282407No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1768930.10087496154096826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT963200.065.913236
CCGCGTT1025350.061.9365847
AGACCGC1019950.061.8909344
CAGACCG1078650.058.8938063
GCGTTCT1238700.051.2072879
TCGACCG899000.050.181778145
GTGCTCT1924000.047.159115145
CGCGTTC1581700.040.0424848
GACCGCG1627200.038.9891625
CTCGCTA584500.038.8278851
TCGCTAT644500.034.659512
ACCGCTA263100.033.555752
TCAGACC2021950.032.5936162
CGCTATG707750.031.5254733
CTCAGAC2418750.029.9540331
CCGCTAA369750.026.1266923
CTTCGAC1194200.023.632322145
CGATCCT1288450.022.968178145
CGCTAAG431350.021.9980414
GGTGCTC1954750.021.11798145
Produced by FastQC (version 0.11.5)