FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175358679
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2339630.13341968662982456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC361050.029.484871
TCAATCG251900.029.323683
AGGGCGA1028750.028.1447684
GCGACAT495350.026.2733387
TGCGACA506850.025.3628796
CGGAGGG1266450.023.7090321
CGACATC681250.019.487058
TACCGTA246600.019.0841269
GGGCGAT349800.018.6412261
GGCGATC348500.018.6241472
CGGCCGC4148850.018.553852145
AATCGCC581550.018.3565882
GGGCGAG1648950.018.1139875
GAGGGCG1632100.018.0643733
GCGAGGC1615650.017.9548977
GGCGAGG1714850.017.5138426
CTGCGAC768550.017.116475
GCTGCGA765400.017.0132564
AGGGATC1156050.016.4795282
CTACCGT300050.015.6818518
Produced by FastQC (version 0.11.5)