FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119983179
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2289620.19082841603988507No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2192260.18271394526061024No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1850370.15421911766481866No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1589950.1325144085405505No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1496150.12469664601902238No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1350890.11258994896276252No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1333010.11109974007273136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT564750.054.0155536
AGACCGC601750.050.4575424
CCGCGTT619150.049.3196687
GTGCTCT1250600.048.692017145
CAGACCG651350.047.1100163
GCGTTCT716950.042.4795349
TCGACCG564300.039.15034145
CGCGTTC856500.035.5583238
TCGCTAT897200.034.3677182
CTCGCTA926000.033.7589151
CGCTATG972700.031.8203073
GACCGCG996800.030.6280885
CGATCCT1679500.025.593431145
CGGTGCT857150.024.953108145
TCAGACC1345500.023.921122
ACCGCTA245050.022.8897362
CCGCTAA259100.022.5568793
GCTATGT1408900.022.3469524
CTCAGAC1636450.022.078251
GTCTCGC875250.021.9444244
Produced by FastQC (version 0.11.5)