FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119983179
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1783630.14865667128223034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG176900.030.9921253
CAATCGC250200.027.6357861
CGGTGGC1840700.025.1550791
TGGCGCG1101000.021.9618474
GCGACAT335900.021.2978747
TGCGACA345250.020.6488276
GGGGACC4827900.020.300919145
GGCGATC242300.019.2200012
AATCGCC360200.019.1776492
GTGGCGC1443400.017.9322433
GGGCGAT260600.017.8260351
AGGGCGA777300.017.3851134
CGGAGGG819900.016.7664151
GGCGCGT1326800.016.607035
GGTCACG631800.016.60057145
CGTTCTT661150.016.562605145
GGTGGCG2232700.015.9847752
CGGCCGC2757300.015.645166145
CGACATC472450.015.5369228
ACGCCCG654150.014.974236
Produced by FastQC (version 0.11.5)