FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132281023
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1709900.1292626834311676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1031500.033.1988564
TCAATCG207700.030.1408083
CAATCGC310000.029.926241
CGGAGGG1197700.028.9487551
TGCGACA388450.025.6486636
GAGGGCG1499900.023.0422823
GGGCGAG1582600.021.9021845
GCGACAT461350.021.8986037
TACCGTA175900.020.7984059
AATCGCC468100.019.8120672
GGGCGAT286800.019.5920491
CGACATC521900.019.5135428
GGCGATC297150.019.114212
GGCGAGG1849050.018.9446096
GCGAGGC1815400.018.7386977
CTACCGT207950.017.6537278
CTGCGAC603550.016.8537985
GGGGACC3823900.016.212349145
GGTCACG582050.016.133152145
GGAGGGC2241750.015.8833222
Produced by FastQC (version 0.11.5)