FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185485449
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA1493600.034.339294
TCAATCG499550.033.908423
CAATCGC727150.031.261061
TGCGACA710550.026.2775826
GCGACAT733400.025.588957
GGCGATC499800.024.668072
TACCGTA310100.023.7908769
GGGCGAT535400.023.1364121
AATCGCC999350.022.8488852
CGGAGGG2338500.022.4683781
GGGCGAG2449900.021.5755585
GCGAGGC2432700.020.8497167
GATCAAT845350.020.7946431
GGCGAGG2543850.020.7837736
ATCAATC937000.019.2299612
CTACCGT397450.018.867618
ACGCCCG1999600.018.6306256
CTCGCGT362100.018.2441378
CGACATC1050900.018.1366318
TTAACGG181350.017.900233
Produced by FastQC (version 0.11.5)