FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92716322
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2290830.24707947323449694No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1081410.11663642136278876No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1068900.11528714437140852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT541600.060.9004756
CCGCGTT578850.056.9703567
AGACCGC577000.056.8741384
CAGACCG614350.053.8338783
GTGCTCT1086800.052.893826145
TCGACCG494850.049.642902145
GCGTTCT687500.047.481039
CGCGTTC848950.038.421098
GACCGCG932400.035.498385
CTCGCTA396400.035.2434581
TCGCTAT419750.032.7381062
CGCTATG427150.032.1289633
ACCGCTA211150.029.3727662
TCAGACC1199800.028.4815252
CCGCTAA229700.028.022933
CTCAGAC1442350.025.9670181
CTTCGAC710050.025.220436145
CGCTAAG253450.024.4632554
CGGTGCT768950.023.31157145
CGATCCT890550.022.944504145
Produced by FastQC (version 0.11.5)