FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92716322
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1038220.11197812613835134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA581300.032.027384
CGGAGGG709950.026.8529381
TCAATCG142900.025.9317323
CAATCGC203100.025.01611
TGCGACA310200.023.4176146
GCGACAT311850.023.3346127
TGGCGCG774150.023.2168624
GGGCGAT213750.023.162541
GGCGATC222300.022.206962
GAGGGCG868950.021.6895393
CGGCCGC2291750.020.988232145
GGGCGAG946250.020.2330155
GCGAGGC955750.019.4916217
CGGTGGC1236950.019.1525881
GGCGAGG1030750.018.6797266
GTGGCGC1040500.018.2361243
CGACATC412800.017.9682048
GGGGACC2661450.017.740864145
AATCGCC311400.016.2625832
AGGGATC644600.015.9896392
Produced by FastQC (version 0.11.5)