FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118109008
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1665410.14100617964719506No Hit
CCCCCCTCGCGGCCCTCCCCCGCCCCACCCCGCGCGCGCCGCTCGCTCCC1320370.11179248918930891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1816550.028.498131
TCAATCG190000.028.3303593
CAATCGC266950.027.9046211
AGGGCGA736400.025.7826844
TGGCGCG1124850.022.4467754
GGGGACC4886750.020.812298145
CGGAGGG970050.020.4932581
GTGGCGC1478400.020.3314693
GGTGGCG2313700.019.2402172
GCGACAT296250.019.053147
TGCGACA294600.019.0074926
AATCGCC408750.018.3596482
GGGCGAG1116950.017.5237165
GCGAGGC1114150.017.1219987
ACGCCCG781850.016.9995486
CGGCCGC3017200.016.915321145
GGCGAGG1225200.016.1316076
GGTCACG669850.015.8579235145
GGCGCGT1450600.015.6166675
TCGCGGC1442000.015.6131727
Produced by FastQC (version 0.11.5)