FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108168429
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2289000.21161442586912307No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1627340.15044500646302258No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1415890.13089678874785174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT448950.067.2112966
AGACCGC491700.061.1537554
CCGCGTT500450.060.256237
TCGACCG508550.057.591763145
CAGACCG541750.056.0260543
GCGTTCT634550.047.4529279
CTCGCTA489350.044.0846671
CGCGTTC718600.041.850218
TCGCTAT524550.040.6440732
GACCGCG754850.040.0740555
CGGTGCT677500.039.213894145
CGCTATG568650.037.374073
GTGCTCT1051700.035.314217145
ACCGCTA144750.029.066452
TCAGACC1210000.026.7933922
CGATCCT1017300.026.631432145
CTCAGAC1472100.025.2256771
CTTCGAC711600.023.242048145
CCGCTAA205800.022.7179623
CCGGTAA125900.019.8805451
Produced by FastQC (version 0.11.5)