FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108168429
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA312050.026.2016286
GCGACAT326250.025.196537
TCAATCG136700.021.2350523
AGGGCGA601000.021.2037354
CAATCGC203650.021.154121
CGGTGGC1245100.020.2090341
CGACATC437400.019.0824438
TACCGTA163950.018.9749079
TGGCGCG815700.018.8015254
CGGAGGG706150.018.747061
GCTGCGA486700.016.9184864
CTGCGAC496000.016.9177975
GGGCGAT222450.016.772611
GGCGATC236500.016.260072
CTACCGT199100.015.9748068
GTGGCGC1086950.014.9007873
GGCGCGT1004800.014.5499925
GGGGACC2912850.014.460286145
GAGGGCG906950.014.3297183
ACTACCG225500.014.2170447
Produced by FastQC (version 0.11.5)