FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005788066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005788066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117705767
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1679120.1426540128658267No Hit
CCCCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGG1657330.14080278666380042No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1222070.10382413972970415No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1201840.10210544739069581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG643950.056.065685145
ACCGCGT318800.051.4033166
CCGCGTT343850.047.6585047
AGACCGC388900.042.0215574
CAGACCG432400.038.4954763
CTCGCTA370750.037.49891
TCGCTAT400550.033.9733962
CGCGTTC497950.032.8172238
CGCTATG430150.031.574913
GCGTTCT539750.030.6894879
GACCGCG566850.028.9211335
ACCGCTA163050.026.9184592
CTTCGAC918800.026.44359145
AGTCGCC433100.025.3563637
TCGCGTC194400.023.7517859
CGATCCT932950.023.553162145
CCGCTAA216750.023.081143
GAGTCGC480200.023.0334386
CTCGCGT207050.021.9516968
AGAGTCG521250.021.5093045
Produced by FastQC (version 0.11.5)